沙冬青种子总黄酮对免疫抑制小鼠免疫功能及胸腺和脾超微结构的影响

doi:10.11843/j.issn.0366-6964.2018.08.021

Abstract

Abstract:

To investigate the effect of total flavonoids from the seeds of Ammopiptanthus mongolicus on the immune function and the ultrastructure of thymus and spleen in immunosuppressed mice,100 Kunming mice were randomly divided into five groups(n=20,half male and half female):normal control group, immunosuppression group (cyclophosphamide induced), test group (cyclophosphamide induced, total flavonoids treated) Ⅰ, test group Ⅱ, and test group Ⅲ, and using intragastric administration of medicine to mice. After 28 days, carbon clearance index, thymus index, spleen index, and the content of IL-2, IL-4 in serum were detected. At the same time, the ultrastructure of thymus and spleen were observed through scanning electron microscope. The results showed that the immunosuppressed mice were copied successfully with cyclophosphamide, and the total flavonoids from the seeds of Ammopiptanthus mongolicus could increase the carbon clearance index, thymus index, and spleen index, the content of IL-2 and IL-4 in serum in immunosuppressed mice. The difference were significantly compared with the immunosuppression group, especially in test group Ⅱ[150 mg·(kg·d)^-1total flavonoids] (P<0.01). Compared with the immunosuppression group, the number of mature lymphocytes in immunosuppressed mice thymus and spleen increased significantly in the total flavonoid-treated groups and the lymphocytes connected closely, especially in test groupⅡ[150 mg·(kg·d)^-1total flavonoids] (P<0.01). The results suggest that the total flavonoids from the seeds of Ammopiptanthus mongolicus can reverse and repair obviously the immune function of the immunosuppressed mice and promote obviously the proliferation and activation of lymphocytes,further more, it has does-dependent relationship with the total flavonoids in a certain extent.

CLC Number:

R282.71

HUANG Wei-kuan, LIU Rui-ju, JIA Ning, FANG Mei, TAO Bo, ZHANG Jia-nan, LIANG Peng-fei, DENG Kang. The Effect of Total Flavonoids from Ammopiptanthus mongolicus on the Immune Function and Ultrastructure of Thymus and Spleen in Immunosuppressed Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1752-1760.

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The Effect of Total Flavonoids from Ammopiptanthus mongolicus on the Immune Function and Ultrastructure of Thymus and Spleen in Immunosuppressed Mice

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